How Does SPAAC Differ from Other Click Chemistry Reactions?
Traditional Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) requires a copper catalyst, which can be cytotoxic and unsuitable for biological applications. In contrast, SPAAC is copper-free, making it particularly valuable for bioconjugation and applications in living systems. The absence of copper reduces potential toxicity and eliminates the need for additional purification steps.
