What are the Commonly Used Methods for Nanotoxicity Testing?
Several in vitro and in vivo methods are employed to assess the toxicity of nanomaterials:
In Vitro Testing: This involves testing on cultured cells to evaluate cytotoxicity, genotoxicity, and oxidative stress. Common assays include the MTT assay, comet assay, and ROS measurement. In Vivo Testing: Animal models such as mice and rats are used to study the systemic effects, biodistribution, and long-term exposure impacts of nanomaterials. Computational Models:In silico methods like Quantitative Structure-Activity Relationship (QSAR) models predict the toxicity of nanoparticles based on their physicochemical properties.
