Various techniques are employed to perform single particle spectroscopy, each with its strengths and limitations:
Fluorescence Microscopy: This technique is widely used to study the optical properties of fluorescent nanoparticles. It involves the use of high-resolution microscopes to detect the emission spectra from individual particles. Raman Spectroscopy: Raman spectroscopy provides information about the vibrational modes of a particle. When combined with advanced optics, it can be used to study single nanoparticles and their chemical compositions. Electron Microscopy: Techniques like Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM) offer high-resolution imaging capabilities that can be paired with spectroscopic methods to analyze individual nanoparticles. Atomic Force Microscopy (AFM) with Spectroscopy: AFM can be combined with spectroscopic techniques to study the mechanical properties of single nanoparticles.
