The ASTM E2524 test involves several steps to assess the cytotoxicity of nanoparticles. Initially, cell cultures are exposed to different concentrations of the nanoparticle under investigation. After a specified incubation period, various assays such as the MTT assay, lactate dehydrogenase (LDH) release, and others are used to measure cell viability and membrane integrity. The results are then analyzed to determine the level of cytotoxicity the nanoparticle exhibits.
