In the field of nanotechnology, the small size and unique properties of nanoparticles can lead to unexpected biological interactions. ASTM E2524 provides a comprehensive framework to evaluate these interactions systematically. This helps in mitigating potential risks associated with nanoparticle usage, ensuring that they are safe for human and environmental exposure. Given the increasing use of nanomaterials in various industries, a standard like ASTM E2524 is indispensable for maintaining safety and efficacy.