To achieve optimal results, consider the following: 1. Speed and Duration: Adjust the vortex mixer’s speed and mixing time to find the balance that provides the best dispersion without damaging the nanoparticles. 2. Volume: Use the appropriate volume for the mixing container to ensure efficient vortex formation. 3. Pre-treatment: For highly agglomerated nanoparticles, pre-treat the solution with ultrasonic waves to break up larger clusters before vortex mixing.