Are There Any Limitations to the Biotin-Streptavidin System?
While the biotin-streptavidin system offers many advantages, it also has some limitations. One potential issue is the possibility of non-specific binding, as streptavidin can sometimes bind to biotin-like molecules present in biological samples. Additionally, the strong binding affinity, while generally beneficial, can make it challenging to dissociate the biotin-streptavidin complex if needed. Furthermore, the introduction of streptavidin or biotin into biological systems can sometimes elicit an immune response, which may limit its use in certain applications.
