Single molecule Förster Resonance Energy Transfer (FRET) is a powerful technique used to study molecular interactions at the nanoscale. It involves the transfer of energy between two fluorescent molecules, a donor and an acceptor, which are in close proximity (typically 1-10 nanometers). The efficiency of this energy transfer is highly sensitive to the distance between the donor and acceptor, making it an excellent tool for measuring nanoscale distances and conformational changes in biological molecules.
