Bio labeling is crucial for several reasons: 1. Enhanced Sensitivity: Nanoparticles such as quantum dots and gold nanoparticles offer higher sensitivity compared to traditional organic dyes. 2. Multiplexing Capability: Different nanoparticles can be engineered to emit various colors, allowing the simultaneous detection of multiple targets. 3. Improved Stability: Nanoparticles exhibit greater photostability compared to conventional dyes, making them suitable for long-term studies.