What is Electrophoretic Mobility Shift Assay (EMSA)?
Electrophoretic Mobility Shift Assay (EMSA) is a powerful technique used to study protein-DNA or protein-RNA interactions. The principle behind EMSA is that a protein bound to a nucleic acid fragment will migrate more slowly through a gel during electrophoresis compared to the unbound nucleic acid. This shift in mobility allows researchers to analyze the binding characteristics and affinities of various proteins for specific nucleic acid sequences.
