Labeling the nucleic acid fragment of interest. Incubating the nucleic acid with the protein of interest to allow binding. Running the mixture on a native polyacrylamide gel. Visualizing the gel to detect shifts in mobility due to binding.
In the context of nanotechnology, the labeled nucleic acids and proteins could be conjugated with nanoparticles to enhance detection sensitivity or to study the effects of nanomaterials on these interactions.
