Despite their significance, cell uptake studies face several challenges:
Complexity of Biological Systems: The dynamic and heterogeneous nature of biological environments complicates nanoparticle behavior prediction. In Vivo vs. In Vitro Discrepancies: Results from cell culture studies may not always translate directly to living organisms. Measurement Sensitivity: Accurately quantifying nanoparticle uptake at low concentrations can be challenging. Long-Term Tracking: Sustained monitoring of nanoparticles within cells over extended periods remains difficult.
Addressing these challenges requires advanced techniques and interdisciplinary approaches.
