What are the challenges associated with AAV Vectors?
Despite their advantages, there are several challenges associated with the use of AAV vectors: 1. Limited packaging capacity: AAV vectors can only carry small genetic payloads (~4.7 kb), which limits their use for larger genes. 2. Pre-existing immunity: Many individuals have pre-existing antibodies against AAV due to natural exposure, which can hinder the efficacy of AAV-based therapies. 3. Production and purification: Large-scale production and high-purity purification of AAV vectors remain technically challenging and costly.
