The test can be performed in both in vitro and in vivo settings. In vitro tests typically involve cultured mammalian cells, while in vivo tests are conducted on model organisms such as rodents. The general procedure includes:
1. Exposure of cells or organisms to the nanomaterial under investigation. 2. Incubation period to allow for cell division. 3. Staining of cells to visualize micronuclei. 4. Microscopic examination to count the number of micronuclei per cell.
