The preparation of heparin loaded nanoparticles involves several steps: 1. Selection of Materials: Choosing the appropriate material for the nanoparticle matrix is critical. Common materials include chitosan, PLGA (poly(lactic-co-glycolic acid)), and lipid-based materials. 2. Encapsulation Techniques: Methods such as emulsion-solvent evaporation, coacervation, and nano-precipitation are employed to encapsulate heparin within the nanoparticles. 3. Surface Modification: The surface of the nanoparticles can be modified with targeting ligands or PEGylation to enhance their specificity and circulation time.
