Several techniques are employed in protein design, including:
1. Computational Modeling: Algorithms predict protein structures and functions based on amino acid sequences. 2. Directed Evolution: Iterative rounds of mutation and selection are used to evolve proteins with desired traits. 3. Synthetic Biology: Custom DNA sequences are synthesized and expressed in cells to produce new proteins. 4. Crystallography and NMR: These techniques help determine the 3D structure of proteins, aiding in the design process.
