size exclusion chromatography (sec)

What Are the Limitations of SEC?


While SEC is a valuable tool, it has some limitations:
Resolution: Limited ability to distinguish between molecules of similar sizes.
Sample Preparation: Requires samples to be soluble in the mobile phase.
Column Fouling: Columns can become clogged with high-concentration samples or aggregates.
Cost: High-quality columns and detectors can be expensive.

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