Imaging techniques are crucial but have inherent limitations:
1. Resolution and Throughput: High-resolution imaging often comes at the cost of lower throughput, which can be a bottleneck for large-scale studies. 2. Depth of Field: Techniques like SEM offer limited depth of field, making it difficult to get a complete picture of three-dimensional nanostructures. 3. Artifacts: Imaging techniques sometimes introduce artifacts, which can be mistaken for actual features unless carefully controlled.