What are the Latest Advances in Nanoscale Imaging?
Recent advancements have significantly enhanced the capabilities of nanoscale imaging:
1. Cryo-Electron Microscopy (Cryo-EM): This technique allows for the imaging of biological specimens in their native state without the need for staining or fixing, providing near-atomic resolution.
2. Super-Resolution Microscopy: Techniques like STED (Stimulated Emission Depletion) microscopy and PALM (Photo-Activated Localization Microscopy) break the diffraction limit of light, achieving resolutions down to tens of nanometers.
3. Correlative Light and Electron Microscopy (CLEM): CLEM combines the advantages of light microscopy and electron microscopy, allowing for the study of dynamic processes in living cells with high resolution.
4. Quantum Dots: These nanocrystals are employed as fluorescent probes in biological imaging, offering high brightness and stability.
