Despite its numerous advantages, the MTS assay also has some limitations:
Interference: Some nanomaterials can interfere with the assay by either reacting with the MTS reagent or absorbing at the same wavelength as the formazan product. Specificity: The assay primarily measures metabolic activity, which may not always correlate with cell viability. Cells under stress may still be metabolically active. Optimization: The assay conditions may need to be optimized for different cell types and nanomaterials.
