To optimize the MTS assay for use with nanomaterials, consider the following steps:
Titration: Perform a titration of the nanomaterial to determine the optimal concentration range for the assay. Controls: Include appropriate controls, such as cells without nanomaterials and nanomaterials without cells, to account for any background interference. Incubation Time: Optimize the incubation time to ensure sufficient formazan production without causing cell death due to prolonged exposure. Validation: Validate the results with complementary assays, such as cell viability assays or live/dead staining, to confirm the findings.
