While SHG offers numerous advantages, it also faces several challenges: 1. Signal Strength: The SHG signal can be weak, especially in small nanostructures, requiring highly sensitive detection equipment. 2. Material Selection: Not all nanomaterials exhibit strong SHG properties; hence, selecting the right material is critical. 3. Complex Interpretation: The interpretation of SHG data can be complex due to the influence of various factors like surface roughness and particle aggregation.