Several challenges exist in conducting in vivo assays for nanotechnology:
- Ethical Concerns: Animal testing raises ethical issues, and there is a growing demand for alternative methods. - Complexity: Biological systems are complex, and it can be difficult to isolate the effects of the nanomaterials from other variables. - Reproducibility: Variability in biological responses can make it challenging to reproduce results consistently. - Translation to Humans: Results from animal studies may not always translate directly to human applications, necessitating further clinical trials.