Conducting in vivo assays typically involves several steps:
1. Selection of Animal Model: Common models include mice, rats, and zebrafish. The choice depends on the research objectives and the specific biological systems of interest. 2. Nanomaterial Administration: Nanoparticles can be administered through various routes such as intravenous, intraperitoneal, oral, or inhalation. 3. Monitoring and Analysis: Researchers monitor physiological responses, biodistribution, and potential toxicological effects using imaging techniques like MRI, CT, and fluorescence microscopy. 4. Data Interpretation: The results are analyzed to understand the interaction of nanoparticles with biological tissues.