Biopersistence is typically assessed through in vitro and in vivo studies. In vitro studies involve exposing nanoparticles to simulated biological fluids and observing their degradation over time. In vivo studies involve monitoring the nanoparticles within living organisms, often using imaging techniques and tracking their clearance from tissues. Additionally, biochemical assays can be used to measure the rate of biodegradation and the potential for bioaccumulation.