How Does Nanopore Sequencing Enhance Transcriptome Analysis?
Nanopore sequencing involves threading RNA molecules through a nanoscale pore. As the RNA passes through the pore, changes in ionic current are measured, allowing for real-time sequencing of RNA. This technology offers several advantages over traditional sequencing methods, including longer read lengths, faster processing times, and the ability to directly sequence RNA without the need for reverse transcription or amplification.