The formulation of LNPs involves several key steps. Initially, lipids are dissolved in a suitable organic solvent. This lipid solution is then mixed with an aqueous phase containing the therapeutic agent. Upon mixing, the lipids self-assemble into nanoparticles, encapsulating the therapeutic agent within. Techniques such as microfluidics and high-pressure homogenization are often employed to control the size and uniformity of the resulting LNPs.
