Correlative Light and Electron Microscopy (CLEM) is an advanced imaging technique that combines the strengths of both
light microscopy and
electron microscopy. Light microscopy allows for live-cell imaging and the observation of fluorescent tags, while electron microscopy provides high-resolution imaging of cellular structures. By integrating these two methods, CLEM offers a comprehensive view of the
nanostructures within biological samples.
CLEM involves a sequential or simultaneous acquisition of images using both light and electron microscopes. The process typically starts with light microscopy to visualize fluorescently labeled structures within a
sample. These images are then correlated with higher-resolution electron microscopy images. Software tools help in aligning and overlaying the images to provide detailed, multi-dimensional information about the sample.
CLEM has a wide range of applications in the field of
nanotechnology, particularly in the area of
biomedical research. It is used to study the
ultrastructure of cells, tissues, and biomaterials at the nanoscale. Some specific applications include:
Drug Delivery: Investigating how nanoparticles interact with cellular structures.
Cancer Research: Studying the distribution of nanoparticles within tumor cells.
Neuroscience: Analyzing synaptic structures and neuron networks.
Material Science: Characterizing nanomaterials and their interactions with biological systems.
The primary advantage of CLEM is its ability to combine the functional information from light microscopy with the structural detail provided by electron microscopy. This dual approach allows for:
High-resolution imaging of cellular structures.
Correlation of dynamic processes with ultrastructural details.
Enhanced understanding of the spatial context of nanomaterials within cells.
Reduced sample preparation artifacts compared to using each technique independently.
Despite its advantages, CLEM also faces several challenges:
Complex Sample Preparation: Preparing samples that are suitable for both light and electron microscopy can be challenging.
Image Correlation: Aligning images from two different microscopy techniques requires sophisticated software and can be time-consuming.
Limited Live Imaging: While light microscopy allows for live imaging, electron microscopy typically requires fixed, dehydrated samples.
Cost: The equipment and expertise required for CLEM can be expensive.
Future Prospects
