Several techniques are employed in protein engineering, including:
Directed Evolution: This technique mimics natural evolution to create proteins with enhanced properties by iterative rounds of mutation and selection. Rational Design: Using computational models, researchers predict how changes in amino acid sequences will affect protein structure and function. CRISPR/Cas9: This genome editing tool is used to introduce precise modifications in the genes encoding proteins. Phage Display: This method involves displaying peptides or proteins on the surface of bacteriophages to screen for specific binding properties.
