Nanofiber scaffolds can be made from a variety of materials, including natural polymers like collagen, chitosan, and silk fibroin, as well as synthetic polymers such as polycaprolactone (PCL), polylactic acid (PLA), and poly(lactide-co-glycolide) (PLGA). The choice of material depends on the intended application and desired properties, such as biocompatibility, biodegradability, and mechanical strength.