A nanofiber scaffold is a three-dimensional structure composed of nanoscale fibers, typically ranging from 50 to 500 nanometers in diameter. These scaffolds are used to mimic the extracellular matrix (ECM) in biological tissues, providing support for cell growth and tissue regeneration. The high surface area to volume ratio of nanofibers enhances cell attachment, proliferation, and differentiation, making them ideal for various biomedical applications.