The future of Cryo-EM in nanotechnology looks promising, with ongoing advancements aimed at overcoming current limitations. Innovations in sample preparation, data processing, and detector technology are expected to enhance the resolution and accessibility of Cryo-EM. As these technologies evolve, Cryo-EM will continue to play a pivotal role in advancing our understanding of nanomaterials and biological systems, driving progress in fields such as biotechnology, medicine, and materials science.