What are the Challenges and Limitations of Cryo-EM?
Despite its advantages, Cryo-EM has some challenges and limitations:
Sample Preparation: Preparing samples for Cryo-EM can be challenging, requiring precise control of freezing conditions to avoid artifacts. Data Processing: The reconstruction of high-resolution 3D models from Cryo-EM images involves complex data processing and analysis, often requiring advanced computational resources. Cost: Cryo-EM equipment is expensive, limiting its accessibility to well-funded research institutions.
