Cryo Scanning Electron Microscopy (Cryo SEM) is a powerful imaging technique used in nanotechnology to observe samples at cryogenic temperatures. This method involves rapidly freezing the sample to preserve its natural state, which is crucial for studying biological materials and other delicate nanostructures. By maintaining the sample at low temperatures, Cryo SEM minimizes the damage caused by the electron beam and prevents the dehydration and shrinkage that can occur in traditional SEM.