Sample Preparation: The sample is rapidly frozen using cryogens such as liquid nitrogen or liquid ethane, ensuring that the ice forms in a vitreous, non-crystalline state. Transfer to Microscope: The frozen sample is transferred to a specialized cryo stage within the scanning electron microscope, which maintains the low temperature throughout the imaging process. Imaging: The electron beam scans the sample, and detectors collect the emitted electrons to create a high-resolution image. The cryogenic environment helps preserve the sample’s morphology and minimizes beam damage.
