Several factors can affect the hydrodynamic radius of nanoparticles: - Solvent viscosity: Higher viscosity results in a larger apparent Rh. - Temperature: Increased temperature generally decreases Rh due to faster Brownian motion. - Particle shape and flexibility: Non-spherical particles and flexible macromolecules have different Rh compared to rigid, spherical particles. - Surface modifications: Functional groups and coatings on nanoparticles can alter their Rh by changing their interaction with the solvent.
