Protein denaturation can be induced by several factors: 1. Temperature: High temperatures can cause proteins to unfold. 2. pH Levels: Extreme pH values can disrupt the ionic and hydrogen bonds holding the protein structure. 3. Chemical Agents: Chemicals like urea or detergents can interfere with the hydrophobic interactions within the protein. 4. Mechanical Forces: Shear forces during mixing or transport can also lead to denaturation.
