What are the Steps Involved in Performing a Resazurin Assay?
Performing a resazurin assay involves several steps:
Preparation of Resazurin Solution: Dissolve resazurin powder in a suitable buffer (e.g., PBS). Cell Seeding: Seed the cells in a 96-well plate and allow them to adhere. Nanomaterial Exposure: Treat the cells with varying concentrations of the nanomaterial. Incubation: After the exposure period, add the resazurin solution to the wells and incubate. Measurement: Measure the fluorescence or absorbance using a microplate reader.
