There are several methods for optical calibration, each suited to different types of instruments and applications:
1. Wavelength Calibration: This method involves the use of known reference wavelengths to ensure that the instrument accurately measures the wavelengths of light. 2. Intensity Calibration: Ensures that the instrument accurately measures the intensity of light, which is critical for applications like fluorescence microscopy. 3. Geometric Calibration: Used for imaging systems to correct any distortions in the captured images, ensuring accurate spatial measurements.
