What are the Limitations of Optical Microscopy in Nanotechnology?
Despite its advantages, optical microscopy has some limitations: - Resolution Limit: The diffraction limit restricts the resolution to about half the wavelength of light used, typically around 200 nm. - Sample Preparation: Some samples require elaborate preparation, which can introduce artifacts or alter the sample. - Depth of Field: Limited depth of field can make it challenging to focus on all parts of a three-dimensional nanostructure simultaneously.
