How is Super-Resolution Microscopy Enhancing Optical Microscopy?
Super-resolution microscopy techniques have revolutionized optical microscopy by breaking the diffraction limit. These techniques include: - STED Microscopy: Uses a depletion beam to quench fluorescence around the target area, allowing for higher resolution. - PALM and STORM: Both rely on the precise localization of individual fluorescent molecules to construct high-resolution images. - SIM: Involves illuminating the sample with patterned light and computationally reconstructing the image to achieve higher resolution.