Despite its advantages, nanoscale imaging has some limitations:
Resolution vs. Sample Damage: Techniques like TEM and SEM require high-energy electrons, which can damage sensitive samples. Sample Preparation: Some methods require extensive sample preparation, which can be time-consuming and may alter the sample's native state. Cost and Complexity: Advanced imaging techniques often require expensive equipment and specialized knowledge to operate. Environmental Conditions: AFM and STM typically require a controlled environment to minimize vibrations and other interferences.
