Several parameters must be considered when conducting a hemolysis assay:
- Nanoparticle Concentration: Varying concentrations of nanoparticles are tested to determine the dose-dependent hemolytic effect. - Incubation Time: The duration of exposure to nanoparticles can influence the extent of hemolysis. - Surface Chemistry: The surface properties of nanoparticles, such as charge and functionalization, can affect their interaction with RBCs. - Size and Shape of Nanoparticles: Different sizes and shapes of nanoparticles may exhibit varying degrees of hemolytic activity.
