The protocol for conducting a hemolysis assay involves several steps:
1. Preparation of RBCs: Blood is collected and centrifuged to isolate RBCs. The RBCs are then washed and resuspended in a suitable buffer. 2. Incubation with Nanoparticles: The RBC suspension is incubated with various concentrations of nanoparticles for a specified period. 3. Centrifugation: After incubation, the samples are centrifuged to separate the intact RBCs from the supernatant. 4. Measurement of Hemoglobin Release: The supernatant is analyzed for hemoglobin release, typically using a spectrophotometer. The degree of hemolysis is quantified by comparing the absorbance of the samples to that of a positive control (complete hemolysis) and a negative control (no hemolysis).
