Toxicity: One of the primary concerns is the potential toxicity of nanomaterials. Studies focus on how these materials interact with biological systems at the cellular and molecular levels. Bioaccumulation: Researchers are investigating whether nanomaterials can accumulate in living organisms, potentially causing long-term environmental and health issues. Persistence: The durability and persistence of nanomaterials in the environment are also areas of concern. These materials may not degrade easily, leading to prolonged exposure and potential harm. Ecotoxicology: The impact of nanomaterials on various environmental compartments, including soil, water, and air, is studied to understand their broader ecological effects.