What are the challenges of using SELEX in nanotechnology?
Despite its advantages, there are several challenges associated with using SELEX in nanotechnology. One major challenge is the time and effort required for the iterative selection and amplification process. Each round of SELEX can take several days, and multiple rounds are often needed to achieve high-affinity aptamers. Additionally, the complexity of the target molecule and the conditions under which binding occurs can affect the success of the SELEX process.
Another challenge is the potential for non-specific binding, which can lead to false positives in sensor applications or off-target effects in drug delivery. Strategies such as counter-selection, where sequences that bind to non-target molecules are removed, can help mitigate this issue, but it remains a significant hurdle.
