What are the Challenges in Using Flow Cytometry for Nanoparticles?
Despite its advantages, there are several challenges associated with using flow cytometry for nanoparticle analysis:
Size limitations: Standard flow cytometers may struggle with detecting very small nanoparticles ( Fluorescent labeling: Requires nanoparticles to be conjugated with fluorescent dyes or markers, which can be complex and time-consuming. Refractive index mismatch: Differences between the refractive indices of nanoparticles and the surrounding fluid can affect accuracy. Data interpretation: Requires sophisticated software and expertise to analyze and interpret the results.
