Flow Cytometry - Nanotechnology

What is Flow Cytometry?

Flow cytometry is a powerful analytical technique that allows for the rapid measurement of physical and chemical characteristics of cells or particles suspended in a fluid. This method uses a laser to excite fluorescently labeled cells or particles as they pass through a detection apparatus. It is widely used in fields such as immunology, hematology, and cell biology.

How is Flow Cytometry Applied in Nanotechnology?

In the context of nanotechnology, flow cytometry has emerged as a critical tool for characterizing and analyzing nanoparticles. It offers high-throughput analysis and can provide detailed information on the size, shape, and surface properties of nanoparticles. This capability is essential for the development and quality control of nanomaterials used in various applications, including drug delivery and biosensing.

What are the Advantages of Using Flow Cytometry for Nanoparticle Analysis?

The primary advantages of using flow cytometry for nanoparticle analysis include:

High-throughput capability: Enables the analysis of thousands of particles per second.
Multiparametric analysis: Allows for simultaneous measurement of multiple characteristics such as size, fluorescence intensity, and surface markers.
Quantitative precision: Provides accurate and reproducible results.
Non-destructive testing: Preserves the integrity of nanoparticles for further analysis.
Flexibility: Can be adapted to study a wide range of nanoparticles with different properties.

What are the Challenges in Using Flow Cytometry for Nanoparticles?

Despite its advantages, there are several challenges associated with using flow cytometry for nanoparticle analysis:

Size limitations: Standard flow cytometers may struggle with detecting very small nanoparticles (

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Assessment of Viability of Listeria monocytogenes by Flow Cytometry.

Issue Release: 2025

Deformability of Heterogeneous Red Blood Cells in Aging and Related Pathologies.

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Flow Cytometry and Automated Microscopy Integration for Phagocytic Analysis of Hypervirulent Klebsiella pneumoniae in Dictyostelium discoideum.

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A Chronological Overview of Using Deep Learning for Leukemia Detection: A Scoping Review.

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The effect of celastrol in combination with 5-fluorouracil on proliferation and apoptosis of gastric cancer cell lines.

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GOLPH3 knockdown alleviates the inflammation and apoptosis in lipopolysaccharide-induced acute lung injury by inhibiting Golgi stress mediated autophagy.

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RhoB plays a central role in hyperosmolarity-induced cell shrinkage in renal cells.

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Ectoine enhances recombinant antibody production in Chinese hamster ovary cells by promoting cell cycle arrest.

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The CCL2-CCR4 Axis Promotes Regulatory T Cell Trafficking to Canine Glioma Tissues.

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The diagnostic value of selected immune parameters in peripheral blood of dogs with malignant mammary tumours - a preliminary study.

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Cataloging circulating CD3CD56 T cells through a series of stimulating (NKG2D, DNAM-1) and inhibitory (PD-1, TIGIT, and Tim-3) immune checkpoint receptors in women diagnosed with precancerous cervical lesions or invasive cervical carcinoma.

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Oxidative stress-induced EGR1 upregulation promotes NR4A3-mediated nucleus pulposus cells apoptosis in intervertebral disc degeneration.

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3-chloro-1,2-propanediol induces oxidative stress and promotes testicular damage and infertility in rats through CYP2C9.

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Clinicopathological differences between T-lymphoblastic leukemia/lymphoma, early T-precursor lymphoblastic leukemia/lymphoma, and mixed-phenotype acute leukemia with T lineage: an analysis of 41 adult cases.

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The Role of LC3-Associated Phagocytosis Inhibits the Inflammatory Response in Aspergillus fumigatus Keratitis.

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Tissue-resident memory CD103+CD8+ T cells in colorectal cancer: its implication as a prognostic and predictive liver metastasis biomarker.

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Identification and validation of tumor-specific T cell receptors from tumor infiltrating lymphocytes using tumor organoid co-cultures.

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Tumor-intrinsic IFNα and CXCL10 are critical for immunotherapeutic efficacy by recruiting and activating T lymphocytes in tumor microenvironment.

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T Helper Cells Producing Granulocyte-Macrophage Colony Stimulating Factor as a Risk Marker for Coronary Heart Disease.

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LINC00665 promotes the progression and immune evasion of lung cancer by facilitating the translation of TCF7 protein through dependence on IRES.

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