What are the Challenges in Using Cas9 in Nanotechnology?
Despite its potential, there are several challenges in using Cas9 in nanotechnology:
Delivery: Efficiently delivering Cas9 to target cells remains a significant hurdle. Off-target Effects: While Cas9 is precise, there is still a risk of off-target genetic modifications. Immune Response: The immune system may recognize Cas9 as a foreign protein, leading to immune reactions. Scalability: Developing scalable and cost-effective methods for producing Cas9-based nanocarriers is challenging.
