What are the Advantages of Multiphoton Excitation?
Multiphoton excitation offers several advantages over traditional single-photon excitation:
Reduced Photodamage: Lower energy photons are less likely to cause photodamage to the specimen. Deeper Penetration: Infrared photons penetrate deeper into biological tissues and other materials. High Spatial Resolution: The nonlinear nature of multiphoton excitation provides better control over the excitation volume, leading to high spatial resolution. Less Background Fluorescence: Nonlinear excitation reduces background fluorescence, improving signal-to-noise ratio.
